Thermo Fisher – Solvents and blends for HPLC applications

INDONESIA

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Indonesia

+62 21 2988 8557

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PHILIPPINES

DKSH Philippines Inc.
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McKinley West, Fort Bonifacio
1634 Taguig City
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+63 2 8548 3200

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VIETNAM

DKSH Technology
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+84 24 3942 4725

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HPLC Brochure
HPLC TFS Food Brochure
HPLC TFS QR Brochure

Our chromatography solvents are made specifically for HPLC instrumentation. They are prepared by a distillation process to remove volatile impurities that can contaminate the analytical columns and reduce their lifetimes, followed by filtration to achieve maximum purity. These solvents have low UV absorption and low peak interference and are free of particles up to 0.02 micron. Thus, usage of our high purity HPLC solvents extends column lifetime. Our HPLC grade solvents are also designed to significantly reduce detector noise and baseline drifts, thereby enhancing data quality and helping ensure reproducibility.

Available Models

Technical Specifications

Description	Packaging	Sizes	Cat. No.
Acetone	Amber Glass Bottles and Safe-Cote™ Bottles (4 L Only)	1 and 4 L	A929
Acetonitrile	Amber Glass Bottles and Safe-Cote™ Bottles (4 L Only)	1 and 4 L	A996
Chloroform (with ~50ppm Amylene as a Preservative)	Amber Glass Bottles	4L	C297
Ethyl Acetate	Amber Glass Bottles and Safe-Cote™ Bottles	4L	E196
Ethyl Acetate/Ethanol 3:1 Solution	Amber Glass Bottles	4L	E151
Hexanes	Amber Glass Bottles and Safe-Cote™ Bottles (4 L Only)	1 and 4 L	H303
n-Hexane, 95%	Amber Glass Bottles and Safe-Cote™ Bottles (4 L Only)	1 and 4 L	H306
Methanol	Amber Glass Bottles and Safe-Cote™ Bottles (4 L Only)	1 and 4 L	A454
Methylene Chloride	Amber Glass Bottles and Safe-Cote™ Bottles (4 L Only)	4L	D151
Petroleum Ether	Amber Glass Bottles and Safe-Cote™ Bottles	4L	E120
2-Propanol	Amber Glass Bottles and Safe-Cote™ Bottles	4L	A464
Tetrahydrofuran	Amber Glass Bottles and Safe-Cote™ Bottles (4 L Only)	1 and 4 L	T427
Toluene	Amber Glass Bottles and Safe-Cote™ Bottles	4L	T291
Water	Amber Glass Bottles and Safe-Cote™ Bottles	4L	W7

Fisher Chemical™ Optima™ grade solvents meet high purity requirements and are the choices for HPLC and GC, spectrophotometry, environmental testing and other analytical applications.

Key Features

Screened for fluorescent and UV-absorbing contaminants
Meet ACS specifications
Certificates of analysis and SDS available online and by scanning the QR codes on the label
Many tested for pesticides via GC ECD

Technical Specifications

Description	Sizes	Cat. No.
Acetic Acid, Glacial (HPLC), Fisher Chemical™	500 mL, 1 L	A/0406
Acetone (HPLC), Fisher Chemical™	1 L, 2.5 L	A/0606
Acetonitrile, for HPLC, Fisher Chemical™	1 L, 2.5 L, 5 L	A/0626
Acetonitrile, HPLC for Gradient Analysis, meets analytical specification of Ph.Eur, Fisher Chemical™	1 L, 2.5 L, 5 L	A/0627
Acetonitrile, Certified, for HPLC-RMN with Low Propionitrile Level	1 L, 2.5 L	A/0636
n-Butyl Chloride (HPLC), Fisher Chemical™	1 L, 2.5 L	C/4756
Chloroform, Stabilized with Amylene, for HPLC, Fisher Chemical™	1 L, 2.5 L	C/4966
Cyclohexane, for HPLC, Fisher Chemical™	1 L, 2.5 L	C/8936
1,2-Dichloroethane, for HPLC, Fisher Chemical™	1 L, 2.5 L	D/1756
Dichloromethane, for HPLC, Stabilised with Amylene, Fisher Chemical™	1 L, 2.5 L, 5 L	D/1856
Dichloromethane, for HPLC, Unstabilised, Fisher Chemical™	1 L, 2.5 L	D/1857
Diethyl Ether, for HPLC, Stabilised with Ethanol, Fisher Chemical™	1 L, 2.5 L	D/2506
DimethylFormamide, for HPLC, Fisher Chemical™	1 L, 2.5 L	D/3846
Dimethyl Sulfoxide, for HPLC, Fisher Chemical™	250 mL, 500 mL, 2.5 L	D/4125
1,4-Dioxane, 99.5+%, for HPLC, Unstabilised, Fisher Chemical™	1 L, 2.5 L	D/4556
Ethanol Absolute, for HPLC, Fisher Chemical™	1 L, 2.5 L	E/0665
Ethyl Acetate, for HPLC, Fisher Chemical™	1 L, 2.5 L	E/0906
Heptane, for HPLC, approx. 99% n-Heptane, Fisher Chemical™	1 L, 2.5 L	H/0106
Heptane, HPLC for fluorescence detection, approx. 99% n-Heptan…	1 L, 2.5 L	H/0107
Isohexane, for HPLC, contains <5% n-Hexane, Fisher Chemical™	1 L, 2.5 L, 5 L, 25 L	H/0405
Hexanes, for HPLC, 95% n-Hexane approx., Fisher Chemical™	1 L, 2.5 L, 5 L	H/0406
Hexanes, HPLC for fluorescence detection, 95% n-Hexane approx., Fisher Chemical™	1 L, 2.5 L	H/0409
Methanol, for HPLC, Fisher Chemical™	1 L, 2.5 L, 5 L	M/4056
Methanol, HPLC for Gradient Analysis, Fisher Chemical™	1 L, 2.5 L, 5 L	M/4058
Methyl-Tert-Butyl Ether, for HPLC, Fisher Chemical™	2.5 L	M/4496
Pentane, for HPLC, Mixed Isomers, Fisher Chemical™	1 L, 2.5 L	P/1006
Propan-1-ol, for HPLC, Fisher Chemical™	1 L, 2.5 L	P/7486
Isopropanol, for HPLC, Fisher Chemical™	1 L, 2.5 L	P/7507
Tetrahydrofuran, for HPLC, Unstabilised, Fisher Chemical™	1 L, 2.5 L	T/0706
Toluene, for HPLC, Fisher Chemical™	1 L, 2.5 L	T/2306
2,2,4-Trimethylpentane (Isooctane), for HPLC, Fisher Chemical™	1 L, 2.5 L, 5 L	T/3606

HPLC grade solvents are specifically for use in analytical and preparatory liquid chromatography.

Key Features

Meet ACS specifications
Submicron filtered
Supplied in specially cleaned bottles
Blanketed with inert gas to maintain integrity
Certificate of Analysis available online
Scan QR codes on label for CofA and SDS
Bulk size options available

Technical Specifications

Description	Packaging	Sizes	Cat. No.
Acetonitrile with 0.1% FA	Amber Glass Bottles	4L	HB9823-4
Acetonitrile with 0.05% TFA	Amber Glass Bottles	4L	HB9812-4
Acetonitrile with 0.1% TFA	Amber Glass Bottles	4L	HB9813-4
Mobile Phase Buffer, HPLC, pH 10, 10mM Ammonium Bicarbonate, 5% Methanol	Amber Glass Bottles	4L	T006024000
Water with 0.1% FA	Amber Glass Bottles	4L	HB523-4
Water with 0.05% TFA	Amber Glass Bottles	4L	HB512-4
Water with 0.1% TFA	Amber Glass Bottles	4L	HB513-4

HPLC mobile phase blends meet the strict purity requirements of HPLC and provide a consistent formulations of formic acid (FA) or trifluoroacetic acid (TFA) with a low LC/UV background. Pre-blended mobile phases offer convenience and consistency in your HPLC operation and avoid the contamination, quality and safety risks often associated with blending solvents and acids in-house.

Key Features

Ready-to-use
Submicron filtered
LC/UV tested at 210nm and 254nm
Lot-to-lot consistency
Packaged in specially treated bottles and sealed with FisherLOCK caps

Improving Separation in Reversed Phase HPLC

High performance liquid chromatography

Application Note

Separation in reversed phase liquid chromatography depends on non-polar analytes being retained by a hydrophobic stationary phase such as a C18 column. If the sample contains ionic (polar) components, they may not be sufficiently retained by the stationary phase for adequate separation. The primary methods of improving retention are via ion pairing or pH modification.

Download Application Note

Key Industries

Aerospace
Agriculture
Automotive
Biopharmaceutical and Biotechnology
Chemical
Construction
Cosmetics & Personal Care
Defense
Die & Mould
Education & Academics
Electronics
Energy
Environmental
Food & Beverage
Luxury
Machinery & Manufacturing
Medical
Mining & Minerals
Oil & Gas
Optical
Paper & Wood
Pharmaceutical
Polymers, Plastics and Rubbers
Powders & Pigments / Coating
Railway
Semiconductor, Solar & Electronics
Service
Shipbuilding
Textile
Wholesale & Retail

Brand

Thermo Fisher Scientific is the world leader in serving science, with revenues of more than $24 billion and approximately 70,000 employees globally. Our mission is to enable our customers to make the world healthier, cleaner and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics, deliver medicines to market and increase laboratory productivity. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services – we offer an unmatched combination of innovative technologies, purchasing convenience and comprehensive services.

Thermo Fisher – Solvents and blends for HPLC applications

INDONESIA

PHILIPPINES

VIETNAM

Available Models

Technical Specifications

Key Features

Technical Specifications

Key Features

Technical Specifications

Key Features

Improving Separation in Reversed Phase HPLC

High performance liquid chromatography

Application Note

Key Industries

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